Actin polyclonal antibody

• Background :

  Beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative :

  ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin; cytoplasmic 2; Gamma-actin

• Swiss Prot :

  P60709/P63261

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC-P, ELISA

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

• Purity :

  Affinity Purified

• Modification :

  Unmodified

• Molecular Weight :

  Calculated MW: 42 kDa; Observed MW: 45 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of Actin in various lysates using Actin antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4