Beta-actin polyclonal antibody

CAT: 0384-BS67001-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS67001-01Size:50 µL
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility. While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids. For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. Research studies have shown that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
Actin cytoplasmic 1; Beta-actin
Swiss Prot
P60709
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Beta-actin. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/3000 - 1/10000), IHC (1/100 - 1/200)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 43 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Beta-actin protein.
Applications Notes
Western blot analysis of Beta-actin expression in HEK293T (A), Hela (B), A375 (C), DLD (D), H460 (E), mouse kidney (F), rat kidney (G) whole cell lysates.
Host or Source
Rabbit

Related Products

CatalogName
MB65882Beta-actin monoclonal antibody
MB66644Beta-actin monoclonal antibody
MB65883-01Beta-actin monoclonal antibody
MB67038Beta-actin monoclonal antibody
MB66645-01Beta-actin monoclonal antibody
MB65885Beta-actin monoclonal antibody
MB65884-01Beta-actin monoclonal antibody
BZ16045-01Actin polyclonal antibody
AP0731β-Actin (D8) polyclonal antibody
BS9201Actin-κ (T121) polyclonal antibody

Alternative Products

CatalogName
E55A04596ACTB/β-actin/Beta Actin Polyclonal Antibody
MBS8519394-01beta-actin Polyclonal Antibody
35-1654-100Polyclonal Antibody to beta-actin
E-AB-40517-01Beta actin Polyclonal Antibody
MBS8591849-01Actin beta Polyclonal Antibody
11-13012-100Polyclonal Antibody to Beta actin
APR00156GPolyclonal Beta-Actin Antibody

Popular Products