Actin (E361) polyclonal antibody

• Background :

  All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  ACTB; Beta-actin; Actin; cytoplasmic 1; β-actin; β actin; βactin; actin-β; actin β, POTEKP, Kappa-actin, ACTBL3, Gamma-actin, ACTG1, ACTG

• Swiss Prot :

  P60709/Q9BYX7/P63261

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids C-terminus of Human Actin.

• Conjugation :

  Unconjugated

• Related Pathways :

  Regulation of Actin Dynamics, Regulation of Actin Dynamics, Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling

• Applications :

  WB IHC

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 45 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Actin (E361) polyclonal antibody detects endogenous levels of Actin protein.

• Applications Notes :

  Western blot (WB) analysis of Actin (E361) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate (40μg) Lane2:HEK293T whole cell lysate (40μg) Lane3:NIH-3T3 whole cell lysate (40μg) Lane4:RAW264.7 whole cell lysate (40μg) Lane5:PC12 whole cell lysate (40μg)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4