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β-Actin (I102) polyclonal antibody

CAT: 0384-AP0060-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP0060-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin
Reactivity
Human, Mouse, Rat, zebrafish, Odontobutis obscurus
Immunogen
Recombinant full length Human β-Actin.
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling
Applications
WB IP IF
Dilution
WB:1:5000~1:20000 IP: 1:100~500 IF: 1:500~1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 42 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
β-actin (I102) polyclonal antibody detects endogenous levels of β-actin protein.
Applications Notes
Western blot (WB) analysis of β-Actin (I102) pAb at 1:5000/1:10000/1:20000 dilution Lane1:CT-26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)
Host or Source
Rabbit

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