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NLRC4 polyclonal antibody

CAT: 0384-BS8872-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS8872-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
LRR; CARD 12; CARD; CARD LRR and NACHT containing protein 1; CARD LRR and NACHT containing protein; CARD12; Caspase recruitment domain containing protein 12; Caspase recruitment domain-containing protein 12; CLAN 1; CLAN A; CLAN; CLAN B; CLAN C; CLAN D; Clan protein; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR 2.1; CLR2.1; ICE protease activating factor; Ice protease-activating factor; Ipaf; NLR family CARD domain containing 4; NLR family CARD domain-containing protein 4; NLRC 4; NLRC4; NLRC4_HUMAN; NACHT-containing protein
Swiss Prot
Q9NPP4
Reactivity
Rat
Immunogen
KLH conjugated synthetic peptide derived from human CARD12:401-500/1024.
Conjugation
Unconjugated
Related Pathways
Inflammasonme Signaling, Necrotic Cell Death
Applications
ELISA IHC ICC IF
Dilution
ELISA 1:5000-10000 IHC 1:100-500 ICC 1:100-500 IF 1:100-500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 116 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NLRC4 polyclonal antibody detects endogenous levels of NLRC4 protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Rat brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NLRC4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Rabbit

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