CD8a Rat monoclonal antibody

• Background:

  Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC) . Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation.

• Structure Composition:

  Rat IgG2a kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

• Product Name Alternative:

  MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a

• Swiss Prot:

  P01731

• Reactivity:

  Mouse

• Immunogen:

  Mouse thymus or spleen

• Conjugation:

  Unconjugated

• Applications:

  IF, FC

• Dilution:

  IF (1/50 - 1/200)

• Purity:

  The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 27 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes mouse CD8a

• Host or Source:

  Rat

• CAS Number:

  9007-83-4