CD8a polyclonal antibody

• Background :

  Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC) . Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation.

• Structure Composition :

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative :

  MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a

• Swiss Prot :

  P01732

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant protein of human CD8a. The exact sequence is proprietary.

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC

• Dilution :

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity :

  The antibody was purified by immunogen affinity chromatography.

• Modification :

  Unmodification

• Molecular Weight :

  ~ 32 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Recognizes endogenous levels of CD8a protein.

• Applications Notes :

  Western blot analysis of CD8a expression in mouse pancreas (A) whole cell lysates.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4