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CD8a Rat Monoclonal Antibody

CAT: 0013-GTR18701870-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18701870-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD8a Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified mouse CD8a .Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a
UniProt
P01731
Reactivity
Mouse
Immunogen
Native purified mouse CD8a
Target
CD8A
Clonality
Monoclonal
Clone
B4X65
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Rat
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Rat IgG2b
Entrez
12525

UniProtKB · P01731

T-cell surface glycoprotein CD8 alpha chain

CD8A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01731
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd8a
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

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