EIF4E monoclonal antibody

• Background:

  The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

• Structure Composition:

  Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

• Swiss Prot:

  P06730

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human EIF4E. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Tumor Angiogenesis, Translational Contral elF4 and p70 S6 Kinase

• Applications:

  WB, IF/ICC

• Dilution:

  WB (1/1000 - 1/2000), IF/ICC (1/10 - 1/50)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 25 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of EIF4E protein.

• Applications Notes:

  Western blot analysis of EIF4E expression in Hela (A), MCF7 (B), K562 (C), NIH3T3 (D), PC12 (E) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4