Phospho-eIF4E (Ser209) polyclonal antibody

• Background:

  EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

• Structure Composition:

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Product Name Alternative:

  EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

• Swiss Prot:

  P06730

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Phospho-eIF4E (S209)

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, ICC/IF, IP

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

• Purity:

  Affinity Purified

• Modification:

  Phosphorylated

• Molecular Weight:

  Calculated MW: 25 kDa; Observed MW: 25 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of eIF4E in HEK293 lysates; mouse spleen lysates using Phospho-eIF4E antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4