EIF4E (T203) polyclonal antibody

• Background:

  EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  EIF4E (phospho S209) ; eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573

• Swiss Prot:

  P06730

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 171-210 of Human eIF4E.

• Conjugation:

  Unconjugated

• Related Pathways:

  Insulin &Glucose Signaling

• Applications:

  WB IHC IP

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 25 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF4E (T203) polyclonal antibody detects endogenous levels of eIF4E protein.

• Applications Notes:

  Western blot (WB) analysis of eIF4E (T203) pAb at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Rat (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4