E2F1 monoclonal antibody

• Background :

  The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative :

  RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

• Swiss Prot :

  Q01094

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Purified recombinant human E2F-1 protein fragments expressed in E.coli.

• Conjugation :

  Unconjugated

• Related Pathways :

  ErbB HER Signaling, Cell Cycle Control G1 S Checkpoin

• Applications :

  WB, IF/ICC, IP

• Dilution :

  WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

• Purity :

  The antibody was purified by immunogen affinity chromatography.

• Modification :

  Unmodification

• Molecular Weight :

  ~ 70 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Recognizes endogenous levels of E2F1 protein.

• Applications Notes :

  Western blot analysis of E2F1 expression in C6 (A), Raw264.7 (B), Hela (C) whole cell lysates.

• Host or Source :

  Mouse

• CAS Number :

  9007-83-4