E2F1 Recombinant Rabbit mAb

• Background :

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

• Structure Composition :

  Store at -20°C. Supplied in 50nM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

• Product Name Alternative :

  RBP3; E2F-1; RBAP1; RBBP3

• Swiss Prot :

  Q01094

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A synthetic peptide of human E2F1

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, ICC/IF, FC, IP

• Purity :

  Affinity Purification

• Modification :

  Unmodification

• Molecular Weight :

  70 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot detection of E2F1 in C6,3T3 cell lysates using E2F1 antibody (1:1000 diluted) .

• Host or Source :

  Rabbit

• CAS Number :

  9000-83-3