E2F1 polyclonal antibody

• Background:

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN

• Swiss Prot:

  Q01094

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human E2F1.

• Conjugation:

  Unconjugated

• Related Pathways:

  ErbB HER Signaling, Cell Cycle Control G1 S Checkpoin

• Applications:

  WB IHC-P ICC/IF IP FC

• Dilution:

  WB: 1:500-1:1000 IHC-P: 1:50-1:100 ICC/IF: 1:50-1:100 IP: 1:20 FC: 1:50-1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 65 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  E2F1 polyclonal antibody detects endogenous levels of E2F1 protein.

• Applications Notes:

  Western blot analysis of E2F1 in (1) HeLa lysates; (2) NIH/3T3 lysates using E2F1 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4