CD61 monoclonal antibody

Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival . The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities . Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) . αIIβ3 and αVβ3 are the two β3 containing integrins which are prominently expressed in hematopoietic cells and angiogenic endothelial cells and perform adhesive functions in hemostasis, wound healing and angiogenesis. Tyr773 and Tyr785 (usually referred to as Tyr747 and Tyr759 based on the chicken sequence) are phosphorylated upon ligand binding . Phosphorylation of these tyrosine residues is required for certain ligand-induced signaling . Thr779 (corresponding to Thr753 of the chicken sequence) of integrin β3 in the platelet specific αIIβ3 is phosphorylated by PKD and/or Akt, which may modulate integrin association with other signaling molecules.

9007-83-4

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61

P05106

Human

Purified recombinant human Integrin beta 3 (N-terminus) protein fragments expressed in E.coli.

Unconjugated

Regulation of Actin Dynamics, MAPK Erk in Growth and Differentiation Pathway, PI3K AKT signaling

WB

WB (1/500 - 1/1000)

The antibody was purified by immunogen affinity chromatography.

Unmodification

~ 100 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

Recognizes endogenous levels of CD61 protein.

Western blot analysis of CD61 expression in Leukocyte whole lysate (A) whole cell lysates.