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PP2C-alpha monoclonal antibody

CAT: 0384-MB66469-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-MB66469-01Size:50 µL
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Background
The α isoform of protein phosphatase 2C (PP2C-α) is the catalytic subunit of a widely expressed serine/threonine phosphatase involved in regulation of the cell stress response. Also known as magnesium-dependent protein phosphatase (PPM1A), this monomeric phosphatase is a member of a conserved group of proteins that acts on many different substrates in numerous pathways. PP2C-α inhibits p38 MAPK and SAPK/JNK pathways activated in response to cell stress as seen in both in vivo and in vitro studies. Specifically, PP2C-α removes phosphates from MKK3 and MKK7, reducing activity of both proteins and inhibiting activation of the downstream kinases JNK and p38 MAPK, respectively. Another PP2C-α substrate is IKKβ, the critical regulator of NF-κB signaling. Dephosphorylation of IKKβ at Ser177/181 by PPM1A and PPM1B results in inactivation of IKKβ and inhibition of NF-κB signaling. PP2C-α is one of the phosphatases responsible for removing phosphate residues from cyclin dependent protein kinases. In a study using HeLa cell extracts, PP2C-α dephospohrylates CDK2 and CDK6, with a preference toward interacting with CDK2 phosphorylated at Thr160, a residue found in the activating T-loop of the kinase. Removal of phosphates from this site is thought to inactivate cyclin-associated kinases. PP2C-α induces cell cycle arrest and apoptosis, likely through activation of p53 though other pathways may also contribute to PP2C-α mediated cell death . Additional PP2C-α substrates include the Wnt signaling pathway protein axin and CFTR, a chloride channel protein implicated in cystic fibrosis.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
Swiss Prot
P35813
Reactivity
Human, Monkey
Immunogen
Recombinant fragment of human PP2C-alpha expressed in E. Coli
Conjugation
Unconjugated
Applications
WB, FC
Dilution
WB (1/500 - 1/1000), FC (1/100 - 1/200)
Purity
The antibody was purified by protein G.
Modification
Unmodification
Molecular Weight
~ 43 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of PP2C-alpha protein.
Applications Notes
Western blot analysis of PP2C-alpha expression in COS7 (A), Jurkat (B), A431 (C), HeLa (D), HEK293 (E), Raji (F), MCF7 (G) whole cell lysates.
Host or Source
Mouse

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