Alpha-enolase monoclonal antibody

• Background:

  Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified: a Enolase, b Enolase and g Enolase. a Enolase expression has been detected on most tissues, whereas b Enolase is expressed predominantly in muscle tissue and g enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

• Structure Composition:

  Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

• Swiss Prot:

  P06733

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant fusion protein of human Alpha-enolase. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 51 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Alpha-enolase protein.

• Applications Notes:

  Western blot analysis of Alpha-enolase expression in MCF7 (A), A431 (B), Hela (C), HepG2 (D), NIH3T3 (E), mouse stomach (F), mouse liver (G) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4