CD169 polyclonal antibody

• Background:

  Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, desig_x0002_nated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) . The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169; SIGLEC1; SN

• Swiss Prot:

  Q9BZZ2

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Recombinant protein of human CD169.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:1000~1:2000 IF 1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 200 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD169 polyclonal antibody detects endogenous levels of CD169 protein.

• Applications Notes:

  Western blot (WB) analysis of CD169 polyclonal antibody at 1:1000 dilution Lane1:RAW264.7 whole cell lysate (30ug) Lane2:Myla2059 whole cell lysate (30ug) Lane3:The Spleen tissue lysate of Rat (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4