CD161 polyclonal antibody

• Background:

  Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha (1,3) Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; CD161

• Swiss Prot:

  Q12918

• Reactivity:

  Rat, Mouse, Human

• Immunogen:

  Recombinant protein of human CD161.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:5000~1:10000 IF 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 43 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD161 polyclonal antibody detects endogenous levels of CD161 protein.

• Applications Notes:

  Western blot (WB) analysis of CD161 polyclonal antibody at 1:5000 dilution Lane1:HEK293T cell membrane lysate (30ug) Lane2:MCF-7 cell membrane lysate (30ug) Lane3:CT-26 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4