CD119 polyclonal antibody

• Background :

  Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation Associates with transmembrane accessory factor IFNGR2 to form a functional receptor.Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription.STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity) .

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative :

  Interferon gamma receptor 1; IFN-gamma receptor; IFN-gamma-R1; CDw119; Interferon gamma receptor alpha-chain; IFN-gamma-R-alpha; CD119

• Swiss Prot :

  P15260

• Reactivity :

  Human, Rat, Mouse

• Immunogen :

  Recombinant protein of human CD119.

• Conjugation :

  Unconjugated

• Applications :

  WB IF

• Dilution :

  WB: 1:1000~1:2000 IF 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 45-90 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CD119 polyclonal antibody detects endogenous levels of CD119 protein.

• Applications Notes :

  Western blot (WB) analysis of CD119 polyclonal antibody at 1:1000 dilution Lane1:The Liver tissue lysate of Rat (30ug) Lane2:SP2/0 cell membrane lysate (30ug) Lane3:HepG2 whole cell lysate (30ug) Lane4:MCF-7 cell membrane lysate (30ug)

• Host or Source :

  Rabbit