CD163 polyclonal antibody

• Background:

  CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type Scavenger Receptor Cysteine-Rich (SRCR) extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding. CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor-associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression, and metastasis. Inflammatory stimulation and stress signals can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163) . The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  M130; CD163; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; sCD163

• Swiss Prot:

  Q86VB7

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Recombinant protein of human CD163.

• Conjugation:

  Unconjugated

• Applications:

  IF

• Dilution:

  IF: 1:100~1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD163 polyclonal antibody detects endogenous levels of CD163 protein.

• Applications Notes:

  Immunofluorescence analysis of MG63 cells using CD163 pAb at dilution of 1:200 (40x lens) .

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4