GOT1 polyclonal antibody

• Background:

  Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  GOT1; AST1; ASTQTL1; GIG18; cAspAT; cCAT

• Swiss Prot:

  P17174

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GOT1.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 46 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  GOT1 polyclonal antibody detects endogenous levels of GOT1 protein.

• Applications Notes:

  Western blot analysis of GOT1 expression in HEK293T (A), Hela (B), DLD (C), mouse heart (D), mouse brain (E), rat heart (F), rat brain (G) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4