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PER2 polyclonal antibody

CAT: 0384-GCP101-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-GCP101-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Biological timepieces called circadian Clocks are responsible for the regulationof hormonal rhythms, sleep cycles and other behaviors. The superchiasmaticnucleus (SCN), which is located in the brain, was the first mammalian circadian Clock to be discovered. A number of transcription factors appearing to bemolecular components of the SCN Clock have been identified. Mutations within the Clock gene increase the length of the endogenous period and cause aloss of rhythmicity of circadian oscillations. Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN. Duringsubjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses. Tim, fortimeless, interacts with Per1 as well as Per2; and Tim and Per1 negativelyregulate Clock-BMAL1-induced transcription.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2; PER2; KIAA0347
Swiss Prot
O15055
Reactivity
Human, Pig
Immunogen
Synthetic peptide, corresponding to Human PER2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:2000~1:5000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 155 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PER2 polyclonal antibody detects endogenous levels of PER2 protein.
Applications Notes
Western blot (WB) analysis of PER2 polyclonal antibody at 1:2000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:The Spleen tissue lysate of Pig (40ug) Lane3:The Lung tissue lysate of Pig (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)
Host or Source
Rabbit

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