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PERK (R87) polyclonal antibody

CAT: 0384-BS2156-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS2156-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK
Swiss Prot
Q9NZJ5
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.
Conjugation
Unconjugated
Related Pathways
Translational Contral Regulation of elF2
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 140 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PERK (R87) polyclonal antibody detects endogenous levels of PERK protein.
Applications Notes
Western blot (WB) analysis of PERK (R87) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:A375 whole cell lysate (20ug) Lane4:CT26 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug)
Host or Source
Rabbit

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