PERK polyclonal antibody

• Background:

  Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) . Involved in control of mitochondrial morphology and function.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

• Swiss Prot:

  Q9NZJ5

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996

• Conjugation:

  Unconjugated

• Applications:

  ICC/IF, WB, IHC-F, IHC-P, ELISA

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 125 kDa; Observed MW: 125 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of PERK in MCF-7 lysates using PERK antibody. The lane on the right is blocked with the synthesized peptide.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4