Cleaved-ADAM12 (E208) polyclonal antibody

• Background:

  ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF (tumor necrosis factor-α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factorlike domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM12 (Meltrin-α) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12; Meltrin-alpha; ADAM12; MLTN; UNQ346; PRO545

• Swiss Prot:

  O43184

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 208-259 of Human ADAM12.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Cleaved

• Molecular Weight:

  ~ 77, 99 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Cleaved-ADAM12 (E208) polyclonal antibody detects endogenous levels of Cleaved-ADAM12 protein.

• Applications Notes:

  Western blot (WB) analysis of Cleaved-ADAM12 (E208) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:The Embryo tissue lysate of Mouse (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4