Cleaved-Notch1 polyclonal antibody

• Background :

  Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative :

  NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1

• Swiss Prot :

  P46531

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784

• Conjugation :

  Unconjugated

• Applications :

  WB, ICC/IF, IHC-F, IHC-P, ELISA

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity :

  Affinity Purified

• Modification :

  Cleaved

• Molecular Weight :

  Calculated MW: 273 kDa; Observed MW: 110 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of Cleaved-Notch1 in various lysates using Cleaved-Notch1 antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4