Cleaved-Notch1 polyclonal antibody

• Background:

  Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1

• Swiss Prot:

  P46531

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784

• Conjugation:

  Unconjugated

• Applications:

  WB, ICC/IF, IHC-F, IHC-P, ELISA

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Cleaved

• Molecular Weight:

  Calculated MW: 273 kDa; Observed MW: 110 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Cleaved-Notch1 in various lysates using Cleaved-Notch1 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4