Cleaved Notch1 polyclonal antibody

• Background:

  This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  AOS5; AOVD1; TAN1; hN1; NOTCH1; Activated NOTCH1; notch 1

• Swiss Prot:

  P46531

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH1.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Cleaved

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Cleaved Notch1 polyclonal antibody detects endogenous levels of Cleaved Notch1 protein.

• Applications Notes:

  Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4