CD63 (K156) polyclonal antibody

• Background:

  CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD63; MLA1; TSPAN30

• Swiss Prot:

  P08962

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 111-160 of Human CD63.

• Conjugation:

  Unconjugated

• Related Pathways:

  Tumor-Infiltrating Immune Cell Marker Guide, Contribution of ECM and Cytoskeletal Factors to EMT

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:2000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 25-60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD63 (K156) polyclonal antibody detects endogenous levels of CD63 protein.

• Applications Notes:

  Western blot (WB) analysis of CD63 (K156) pAb at 1:1000 dilution Lane1:C6 whole cell lysate (30ug) Lane2:SP2/0 whole cell lysate (30ug) Lane3:K562 whole cell lysate (30ug) Lane4:A549 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4