Insulin Antibody / INS

• Description:

  Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

• Specifications:

  Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL (No special pretreatment is required for staining of FFPE tissue)

• UniProt:

  P01308

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human Insulin protein was used as the immunogen for the Insulin antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RIRDN/8546

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Insulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Insulin antibody is available for research use only.

• Storage Conditions:

  Aliquot the Insulin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Insulin antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human pancreas tissue with Insulin antibody (clone rIRDN/8546) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.