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Insulin Antibody

CAT: 0800-R30045Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30045Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Insulin, synthesized by the beta cells of the islets of Langerhans, consists of two dissimilar polypeptide chains, A and B, which are linked by two disulfide bonds. The gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL
Host
Mouse
Reactivity
Human, Rabbit, Rat
Immunogen
Human insulin was used as the immunogen for this Insulin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
KC36AC10
Applications
IHC-P
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Insulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Insulin antibody is available for research use only.
Storage Conditions
After reconstitution, the Insulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Insulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC-P testing of rat pancreas tissue with insulin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

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