TRPC6 Antibody

• Description:

  Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate, and is likely to be involved in the formation of the hydroxylating intermediate. TPH is both the first and rate-limiting-step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, LDOPA, which is used as a common therapy for Parkinson s disease (PD) patients, inhibits TPH function which, subsequently, is thought to contribute to the onset of depression in PD patients.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  Q9Y210

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human TRPC6 protein was used as the immunogen for the TRPC6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2 κ

• Clone:

  TRPC6/7671

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the TRPC6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This TRPC6 antibody is available for research use only.

• Storage Conditions:

  Aliquot the TRPC6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TRPC6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC staining of FFPE human pancreas tissue with TRPC6 antibody (clone TRPC6/7671) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.