SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit

SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit

• Abbreviation: SARS-CoV-2 IgM
• Synonyms: COVID 19 IgM;N protein IgM;
• CAS Number: 7732-18-5
• Species Reactivity: Human
• Type: Indirect-ELISA
• Applications: ELISA
• Field of Research: SARS-CoV-2;COVID 19
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.
• Assay Performance Time: 1 h 30 min
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months