SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit

• Abbreviation:

  SARS-CoV-2 IgM

• Cross Reactivity:

  This kit recognizes Human SARS-CoV-2 IgM in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 IgM and analogues was observed

• Target:

  COVID 19 IgM; N protein IgM

• Type:

  Indirect-ELISA

• Applications:

  ELISA

• Field of Research:

  SARS-CoV-2; COVID 19

• Assay Principle:

  This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.

• Assay Performance Time:

  1 h 30 min

• Sample Type:

  Serum, plasma

• Precision:

  Intra-CV: CV%＜8%; Inter-CV: CV%＜10%

• Recovery:

  80%-120%

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0956566321005728

• Detection Instrument:

  Microplate reader

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.