Recombinant SARS-CoV-2 Nucleocapsid Protein, Biotinylated (His Tag)

Recombinant SARS-CoV-2 Nucleocapsid Protein, Biotinylated (His Tag)

• Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• Synonyms: 2019-nCoV coronavirus NP Protein, 2019-nCoV novel coronavirus Nucleoprotein Protein, 2019-nCoV np Protein, NP
• CAS Number: 9000-83-3
• Accession Number: YP_009724397.2
• Host: Baculovirus
• Origin Species: SARS-CoV-2
• Tag: C-His
• Sequence: Met1-Ala419(335Gly/Ala)
• Purity: > 85 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.