Recombinant SARS-CoV-2 Nucleocapsid Protein (His Tag)

• Background:

  Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

• Abbreviation:

  Nucleocapsid

• Synonyms:

  2019-nCoV coronavirus NP Protein;2019-nCoV novel coronavirus Nucleoprotein Protein;2019-nCoV np Protein

• UniProt:

  P0DTC9

• Accession Number:

  P0DTC9

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met1-Ala419

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  49.4 kDa

• Observed Molecular Weight:

  50-60 kDa

• Species:

  SARS-CoV-2