Recombinant SARS-CoV Nucleocapsid Protein

• Background:

  Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

• Abbreviation:

  Nucleocapsid

• Synonyms:

  SARS-CoV coronavirus NP Protein; SARS-CoV novel coronavirus Nucleoprotein; SARS-CoV np Protein

• UniProt:

  P59595

• Accession Number:

  P59595

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Met1-Ala422

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 85 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  49.7 kDa

• Observed Molecular Weight:

  58 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5

• Species:

  SARS