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BCECF-AM

CAT: 0804-HY-101883-01Size: 205 μg (5 mM x 50 μL in DMSO)Dry Ice: NoHazardous: No
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CAT#:0804-HY-101883-01Size:205 μg (5 mM x 50 μL in DMSO)
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH. BCECF-AM could diffuse through the cell membrane and intracellular esterase cleave the ester bond releasing BCECF. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
CAS Number
117464-70-7
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/BCECF-AM.html
Concentration
5 mM * 200 μL in DMSO
Purity
99.0
Solubility
10 mM in DMSO
Smiles
O=C(OCOC(C)=O)CCC1=CC(C23OC(C4=C2C=CC(C(OCOC(C)=O)=O)=C4)=O)=C(OC5=C3C=C(CCC(OCOC(C)=O)=O)C(OC(C)=O)=C5)C=C1OC(C)=O.O=C(OCOC(C)=O)CCC6=CC(C78OC(C9=C7C=C(C(OCOC(C)=O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(OCOC(C)=O)=O)C(OC(C)=O)=C%10)C=C6OC(C)=O
Molecular Formula
C40H36O19
Molecular Weight
820.70
Precautions
H315, H319, H335
References & Citations
[1]Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7 (9) : e46303.|[2]Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis- (2-carboxyethyl) -5 (and-6) -carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76 (2) :139-45.|[3]Chen F, et al. CYP51A1 drives resistance to pH-dependent cell death in pancreatic cancer. Nat Commun. 2025 Mar 7;16 (1) :2278. |[4]Pang X, et al. Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration. Nat Commun. 2023 Jul 14;14 (1) :4207.
Shipping Conditions
Blue Ice
Storage Conditions
Solution, -20°C, protect from light, 2 years
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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