BCECF Acetoxymethyl ester

• Description:

  BCECF Acetoxymethyl ester is a fluorescent indicator for estimating intracellular pH. BCECF Acetoxymethyl ester is modified by intracellular esterases to produce BCECF. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm[1][2][3].

• UNSPSC:

  12352005

• Target:

  Fluorescent Dye

• Related Pathways:

  Others

• Field of Research:

  Others

• Smiles:

  O=C(C1=CC=C2C(C(OC23C4=C(OC5=C3C=C(CCC(OCOC(C)=O)=O)C(OCOC=O)=C5)C=C(OCOC(C)=O)C(CCC(OCOC(C)=O)=O)=C4)=O)=C1)OCOC(C)=O

• Molecular Formula:

  C41H38O21

• Molecular Weight:

  866.73

• References & Citations:

  [1]Bachmeier CJ, et al. Drug efflux transport properties of 2',7'-bis (2-carboxyethyl) -5 (6) -carboxyfluorescein acetoxymethyl ester (BCECF-AM) and its fluorescent free acid, BCECF. J Pharm Sci. 2004 Apr;93 (4) :932-42.|[2]Dive C, et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol Cell Probes. 1988 Jun;2 (2) :131-45.|[3]O'Connor N, et al. Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol. 2007;81:415-33.

• Shipping Conditions:

  Room temperature

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  [1119449-23-8]