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DMPO Antibody (APC)

CAT: 0013-GTR18028562Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18028562Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse monoclonal to DMPO (APC) . The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6) . Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals..
Product Name Alternative
5,5-dimethyl-1-pyrroline N-oxide, 5,5-dimethyl-2- (8-octanoic acid) -1-pyrroline N-oxide, DMPO nitrone adduct, 55 dimethyl 1 pyrroline N oxide nitrone adduct
Reactivity
All
Immunogen
5,5-dimethyl-2- (8-octanoic acid) -1-pyrrolone-N-oxide conjugated to Ovalbumin
Target
DMPO
Clonality
Monoclonal
Clone
N1664A
Conjugation
APC
Field of Research
Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction
Purification
Protein G Purified
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25μg)
Storage Conditions
Conjugated antibodies should be stored according to the product label
Notes
For research use only.
Applications Notes
A 1:1000 dilution was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Tested Applications
AM, ELISA, ICC, IF, IHC, IP, WB
Host or Source
Mouse
Preservative
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Isotype
IgG1

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