Rab5 Antibody

• Background:

  Rab5 is a 24 kDa GTPase that regulates early endosome dynamics and endocytic trafficking. It is ubiquitously expressed and localizes to early endosomes and the plasma membrane. Rab5 controls the fusion of endocytic vesicles with early endosomes and mediates homotypic endosome fusion. It recruits effectors such as EEA1 and Rabaptin-5 to facilitate vesicle docking and fusion. In the nervous system, Rab5 is essential for receptor internalization, synaptic vesicle recycling, and neurotrophin signaling. Dysregulation of Rab5 activity can lead to endosomal dysfunction, impaired signaling, and neurodegeneration. It is widely used as a marker of early endosomes and is a key player in maintaining neuronal homeostasis.

• Description:

  Rabbit Anti-Human Rab5 Polyclonal

• Specifications:

  Detects ~26kDa.

• Product Name Alternative:

  Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  5868

• Swiss Prot:

  P20339

• Accession Number:

  NP_004153.2

• Cellular Locus:

  Cell Membrane | Endosome | Early Endosome Membrane | Melanosome

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat, Bovine, Monkey

• Immunogen:

  Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues

• Target:

  Rab5

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF

• Validated Applications:

  WB, IHC, ICC/IF

• Field of Research:

  Cell Signaling | Tags and Cell Markers | Organelle Markers | Protein Trafficking | Vesicle Transport| Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

• Purification:

  Protein A Purified

• Detection Range:

  1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:100), ICC/IF (1:80) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~26kDa.

• Species:

  Human | Mouse | Rat | Bovine | Monkey

• CAS Number:

  9007-83-4

• Location:

  Cell Membrane | Endosome | Early Endosome Membrane | Melanosome

• Immunogen Species:

  Human