RegisterLogin

Rab5 Antibody

CAT: 0400-SPC-168SSize: 12 µgDry Ice: NoHazardous: No
CAT#:0400-SPC-168SSize:12 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Rab5 is a 24 kDa GTPase that regulates early endosome dynamics and endocytic trafficking. It is ubiquitously expressed and localizes to early endosomes and the plasma membrane. Rab5 controls the fusion of endocytic vesicles with early endosomes and mediates homotypic endosome fusion. It recruits effectors such as EEA1 and Rabaptin-5 to facilitate vesicle docking and fusion. In the nervous system, Rab5 is essential for receptor internalization, synaptic vesicle recycling, and neurotrophin signaling. Dysregulation of Rab5 activity can lead to endosomal dysfunction, impaired signaling, and neurodegeneration. It is widely used as a marker of early endosomes and is a key player in maintaining neuronal homeostasis.
Description
Rabbit Anti-Human Rab5 Polyclonal
CAS Number
9007-83-4
Specifications
Detects ~26kDa.
Product Name Alternative
Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
5868
Swiss Prot
P20339
Accession Number
NP_004153.2
Cellular Locus
Cell Membrane | Endosome | Early Endosome Membrane | Melanosome
Host
Rabbit
Species Reactivity
Human
,
Mouse
,
Rat
,
Bovine
,
Monkey
Immunogen
Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Target
Rab5
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal
Applications
WB | IHC | ICC/IF
Validated Applications
WB
,
IHC
,
ICC/IF
Field of Research
Cell Signaling | Tags and Cell Markers | Organelle Markers | Protein Trafficking | Vesicle Transport| Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers
Purification
Protein A Purified
Detection Range
1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000), IHC (1:100), ICC/IF (1:80) ; optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Detects ~26kDa.
Species
Human | Mouse | Rat | Bovine | Monkey
Location
Cell Membrane | Endosome | Early Endosome Membrane | Melanosome
Immunogen Species
Human

Related Products

CatalogName
SPR-121CRab5 Protein
SPR-121ARab5 Protein
SPR-121BRab5 Protein
SPC-141SRab4 Antibody
SPC-141DRab4 Antibody
SPC-141D-RPERab4 Antibody: RPE
SPC-141D-FITCRab4 Antibody: FITC
SPC-141D-APCRab4 Antibody: APC
SPC-141D-HRPRab4 Antibody: HRP
SPC-141D-PCPRab4 Antibody: PerCP

Alternative Products

CatalogName
MBS5309878-01RAB5 antibody
MB11494Rab5 monoclonal antibody
BZ16636Rab5 polyclonal antibody
56415Anti-Rab5 Antibody
MBS805854-01Rab5 Antibody: RPE
MBS9456631-01RAB5 Conjugated Antibody
E8ET1609-27Rab5

Popular Products