Rab4 Antibody: FITC

• Background:

  Rab4 is a 25 kDa GTPase of the Ras superfamily that regulates early endosomal recycling. It cycles between an inactive GDP-bound state and an active GTP-bound state, recruiting effector proteins to intracellular membranes. Rab4 modulates receptor recycling, including transferrin and glucose transporter (GLUT4) trafficking. Overexpression of Rab4 alters receptor distribution, leading to accumulation in post-endosomal compartments. Its activity is regulated by phosphorylation and post-translational lipid modifications, which influence membrane association and subcellular localization. In neurons, Rab4 is involved in synaptic vesicle recycling and receptor trafficking. Dysregulation of Rab4 may impair endosomal sorting and contribute to synaptic dysfunction in neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

• Description:

  Rabbit Anti-Human Rab4 Polyclonal

• Specifications:

  Detects ~26kDa.

• Product Name Alternative:

  Oncogene Rab4, Rab4A, Ras related protein

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  5867

• Swiss Prot:

  P20338

• Accession Number:

  NP_004569.2

• Cellular Locus:

  Membrane | Cytoplasm

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  C-terminal peptide from human Rab4

• Target:

  Rab4

• Clonality:

  Polyclonal

• Conjugation:

  FITC

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Cell Signaling | Tags and Cell Markers | Organelle Markers | Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

• Purification:

  Peptide Affinity Purified

• Detection Range:

  A 1:1000 dilution of SPC-141 was sufficient for detection of Rab4 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:100), ICC/IF (1:150) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11 (12), 4379-4389.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~26kDa.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Membrane | Cytoplasm

• Immunogen Species:

  Human