HSP90 alpha/beta Antibody, Clone Hyb-K41220A

• Background:

  HSP90α and HSP90β are the two major cytosolic isoforms of the highly conserved heat shock protein 90 (HSP90) family, essential for maintaining protein homeostasis in all eukaryotic cells. While they share 85% amino acid sequence identity, the isoforms differ in structure and function: HSP90α primarily forms homodimers and is stress-inducible, whereas HSP90β exists mainly as a monomer and is constitutively expressed. Both isoforms are abundantly expressed in the brain and play critical roles in the folding, maturation, and stabilization of a wide range of client proteins involved in neuronal signaling, synaptic plasticity, and cellular stress responses. These include kinases (e.g., c-Raf), transcription factors (e.g., p53), and hormone receptors—many of which are implicated in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease. HSP90α and HSP90β function as part of dynamic chaperone complexes, interacting with co-chaperones like Cdc37 and p23. ATP binding drives conformational changes that enable these complexes to stabilize client proteins and prevent their degradation. However, in disease contexts, this protective mechanism can inadvertently preserve toxic protein species, contributing to pathogenesis. By recognizing both HSP90α and HSP90β, this antibody provides a powerful tool for researchers exploring the dual roles of HSP90 in neuronal health and disease.

• Description:

  Mouse Anti-Human HSP90 alpha/beta Monoclonal IgG2a

• Specifications:

  Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.

• Product Name Alternative:

  HSP90AA1, HSP90AB1, HSP90-alpha, HSP90-beta, HSP90A, HSP90B, HSP89A, HSP86, HSP84, HSP90Alpha, HSPCA, HSPC1, HSPC2, HSPCB, HSPCAL3, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Heat shock 90 kDa protein 1 alpha isoform, Heat shock 84 kDa protein, D6S182, FLJ26984

• Synonyms:

  HSP90 alpha/beta Antibody, Clone Hyb-K41220A

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  3326, 3320

• Swiss Prot:

  P08238, P07900

• Accession Number:

  NP_031381.2, NP_001017963.2

• Cellular Locus:

  Cytoplasm | Melanosome

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat, Yeast

• Immunogen:

  Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304

• Target:

  HSP90 alpha/beta

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  Hyb-K41220A

• Conjugation:

  Unconjugated

• Type:

  Monoclonal

• Applications:

  WB | IHC | ICC/IF | ELISA

• Validated Applications:

  WB, IHC, ICC/IF, ELISA

• Field of Research:

  Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.2, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4): 296-311.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.

• Background Reference 01:

  1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Kishimoto J, et al. (2005) . Cell Stress and Chaperones. 10 (4) : 296-311.

• Species:

  Human | Mouse | Rat | Yeast | Yeast (Saccharomyces cerevisiae) | Fisson Yeast (Schizosaccharomyces pombe)

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Melanosome

• Isotype:

  IgG2a

• Immunogen Species:

  Human