Coelenteramine 400a

• Description :

  Coelenteramine 400a (Coelenterazine 400a), a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate. In the presence of Coelenteramine 400a, RLuc can emit blue light at 395 nm[1][2]. Coelenterazine 400a will causes color change in the bioluminescence reaction of Rluc by replacing the sulfur and oxygen heteroatoms of the methylene bridge. Coelenterazine 400a provides higher signal resolution and can be used in the research of bioluminescence resonance energy transfer (BRET) [3].

• Product Name Alternative :

  Coelenterazine 400a; Bisdeoxycoelenterazine

• UNSPSC :

  12171500

• Hazard Statement :

  H302, H315, H319, H335

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/coelenteramine-400a.html

• Purity :

  99.70

• Solubility :

  DMSO : 8.33 mg/mL (ultrasonic) |Ethanol : 1 mg/mL (ultrasonic; warming; heat to 60°C)

• Smiles :

  O=C1C(CC2=CC=CC=C2)=NC3=C(NC(C4=CC=CC=C4)=CN31)CC5=CC=CC=C5

• Molecular Formula :

  C26H21N3O

• Molecular Weight :

  391.46

• Precautions :

  H302, H315, H319, H335

• References & Citations :

  [1]Levi J, et, al. Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays. J Am Chem Soc. 2007 Oct 3;129 (39) :11900-1. |[2]Bertrand L, et, al. The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS) . J Recept Signal Transduct Res. Feb-Nov 2002;22 (1-4) :533-41.|[3]Yuan M L, et al. Luminescence of coelenterazine derivatives with C-8 extended electronic conjugation[J]. Chinese Chemical Letters, 2016, 27 (4) : 550-554.|[4]Ikeda Y, et al. Quantitative evaluation of luminescence intensity from enzymatic luminescence reaction of coelenterazine and analogues. Journal of Photochemistry and Photobiology A: Chemistry, 2020, 394: 112459.

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  -20°C (Powder, protect from light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [70217-82-2]