Coelenterazine

• UNSPSC Description:

  Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite[1][2][3].

• Target Antigen:

  Reactive Oxygen Species

• Type:

  Dye Reagents

• Related Pathways:

  Immunology/Inflammation;Metabolic Enzyme/Protease;NF-κB

• Applications:

  Cancer-programmed cell death

• Field of Research:

  Cancer

• Assay Protocol:

  https://www.medchemexpress.com/Coelenterazine.html

• Purity:

  98.94

• Solubility:

  DMSO : 50 mg/mL (ultrasonic)|Ethanol : 1 mg/mL (ultrasonic;warming;heat to 60°C)

• Smiles:

  O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31

• Molecular Weight:

  423.46

• References & Citations:

  [1]Markova SV, et al, Coelenterazine-dependent luciferases. Biochemistry (Mosc). 2015 Jun;80(6):714-32.|[2]Lucas M, et al. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils. Anal Biochem. 1992 Nov 1;206(2):273-7.|[3]Pichler A, et al. In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein. Clin Cancer Res. 2005 Jun 15;11(12):4487-94.|[4]Volk-Draper LD, et al. Novel model for basaloid triple-negative breast cancer: behavior in vivo and response to therapy. Neoplasia. 2012 Oct;14(10):926-42.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, protect from light, stored under nitrogen)

• Clinical Information:

  No Development Reported

• CAS Number:

  55779-48-1