AChRα10 Polyclonal Antibody

Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα10, also known as CHRNA10, is a 450 amino acid multi-pass membrane protein expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes. AChRα10 forms a hetero-oligomeric channels in conjunction with AChRα9 and is considered an ionotropic receptor with a probable role in the modulation of auditory stimuli.

Rabbit Anti-AChRα10 Polyclonal Antibody

9007-83-4

CHRNA10; NACHRA10; Neuronal acetylcholine receptor subunit alpha-10; Nicotinic acetylcholine receptor subunit alpha-10; NACHR alpha-10

57053

Q9GZZ6

Human, Mouse, Rat

Synthesized peptide derived from the C-terminal region of human AChRα10 at AA range: 370-450

Neuronal acetylcholine receptor subunit alpha-10

WB, ELISA

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

50kD

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Gel pack with blue ice.

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

AChRα10 Polyclonal Antibody detects endogenous levels of AChRα10 protein.

Liquid solution

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.

CHRNA10; NACHRA10; Neuronal acetylcholine receptor subunit alpha-10; Nicotinic acetylcholine receptor subunit alpha-10; NACHR alpha-10

WB, IHC, IF, IP, ELISA

Rabbit

http://www.uniprot.org/uniprot/Q9GZZ6