LIMA1 rabbit pAb

• Background:

  This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

• Description:

  This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011],

• Gene ID:

  51474

• UniProt:

  Q9UHB6

• Cellular Locus:

  Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell membrane. Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681). Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988). Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity)..

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse,

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Synthesized peptide derived from part region of human protein AA range: 631-681

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA

• Validated Applications:

  WB, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-2000 ELISA 1:5000-20000

• Molecular Weight:

  83kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  83 kD

• Subcellular Location:

  Cytoplasm. Cell junction, focal adhesion . Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber . Cell membrane . Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681) . Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988) . Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity) . .

• Gene ID (Human):

  51474

• SwissProt (Human):

  Q9UHB6