Golgin 45 rabbit pAb

CAT: 0855-ES7816-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0855-ES7816-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing., function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface., induction:Up-regulated by retinoids., subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi., tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
Description
Caution: Because of the presence of a potential basic motif and leucine-zipper domain, PubMed: 9129147 and PubMed: 11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing. function: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. induction: Up-regulated by retinoids. subunit: Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi. tissue specificity: Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
UniProt
Q9H2G9
Swiss Prot
Q9H2G9
Reactivity
Human; Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
47kD
Storage Conditions
-20°C/1 year
Observed Molecular Weight
47kD
Fragment
IgG
Subcellular Location
Golgi apparatus membrane .; [Isoform 1]: Nucleus . Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm. .; [Isoform 2]: Cytoplasm . Not detected in the nucleus. .
Other Product Names
BLZF1; JEM1; Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor
Gene ID (Human)
8548

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