RFWD3 rabbit pAb

• Background:

  PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 1 RING-type zinc finger., similarity:Contains 3 WD repeats.

• Description:

  PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Contains 1 RING-type zinc finger. similarity: Contains 3 WD repeats.

• UniProt:

  Q6PCD5

• Swiss Prot:

  Q6PCD5

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  85kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  85kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276) . In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657) . This recruitment depends upon RPA2 (PubMed:21504906) . .

• Other Product Names:

  RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201

• Gene ID (Human):

  55159