SPARC rabbit pAb

• Description:

  This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],

• Synonyms:

  SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

• Gene ID:

  6678

• UniProt:

  P09486

• Cellular Locus:

  Secreted, extracellular space, extracellular matrix, basement membrane. In or around the basement membrane.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190

• Clonality:

  Polyclonal

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight:

  34kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.