MYSM1 rabbit pAb

MYSM1 rabbit pAb

• Description: Catalytic activity: Ubiquitin C-terminal thioester + H (2)O = ubiquitin + a thiol. Domain: Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA. function: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Belongs to the peptidase M67A family. MYSM1 subfamily. similarity: Contains 1 MPN (JAB/Mov34) domain. similarity: Contains 1 SANT domain. similarity: Contains 1 SWIRM domain. subunit: Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones.
• Synonyms: MYSM1; KIAA1915; Histone H2A deubiquitinase MYSM1; 2A-DUB; Myb-like; SWIRM and MPN domain-containing protein 1
• Gene ID: 114803
• UniProt: Q5VVJ2
• Cellular Locus: Nucleus. Cytoplasm. Localizes to the cytoplasm in response to bacterial infection..
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human MYSM1. AA range:520-569
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 95kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.